Establishment of integration free iPSC clones, NCCSi011-A and NCCSi011-B from a liver cirrhosis patient of Indian origin with hepatic encephalopathy

Arun Vaidyanath; Mohsina Khan; Bhumika Vaishnav; Arjun Lal Kakrani; Swapnil Patil; Anjali Shiras

doi:10.1016/j.scr.2020.101992

Establishment of integration free iPSC clones, NCCSi011-A and NCCSi011-B from a liver cirrhosis patient of Indian origin with hepatic encephalopathy

Stem Cell Res. 2020 Oct:48:101992. doi: 10.1016/j.scr.2020.101992. Epub 2020 Sep 15.

Authors

Arun Vaidyanath¹, Mohsina Khan¹, Bhumika Vaishnav², Arjun Lal Kakrani², Swapnil Patil², Anjali Shiras³

Affiliations

¹ National Centre for Cell Science (NCCS), SP Pune University Campus, Ganeshkhind, Pune 411007, Maharashtra, India.
² Dr. D.Y. Patil Medical College, Hospital and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pune 411018, Maharashtra, India.
³ National Centre for Cell Science (NCCS), SP Pune University Campus, Ganeshkhind, Pune 411007, Maharashtra, India. Electronic address: anjali@nccs.res.in.

PMID: 32971460
DOI: 10.1016/j.scr.2020.101992

Abstract

Liver cirrhosis accompanied with hepatic encephalopathy commonly causes cognitive impairment in patients. To model this disease, two independent patient specific induced pluripotent stem cell-line (iPSC) clones, NCCSi011-A and NCCSi011-B were generated by reprogramming the CD4⁺ T cells of an Indian male patient suffering from this chronic condition. Both clones expressed the stemness markers, formed embryoid bodies (EBs) with potential for spontaneous differentiation in to all the three lineages, exhibited normal karyotype (46, XY) and demonstrated alkaline phosphatase activity. These generated iPSC lines have potential for use in understanding biology of the disease and evaluation of drugs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Clone Cells
Embryoid Bodies
Hepatic Encephalopathy*
Humans
Induced Pluripotent Stem Cells*
Liver Cirrhosis
Male