Super-resolution imaging of bacterial pathogens and visualization of their secreted effectors

FEMS Microbiol Rev. 2021 Mar 16;45(2):fuaa050. doi: 10.1093/femsre/fuaa050.

Abstract

Recent advances in super-resolution imaging techniques, together with new fluorescent probes have enhanced our understanding of bacterial pathogenesis and their interplay within the host. In this review, we provide an overview of what these techniques have taught us about the bacterial lifestyle, the nucleoid organization, its complex protein secretion systems, as well as the secreted virulence factors.

Keywords: Salmonella; Shigella; Vibrio; EnvZ; OmpR; PAINT; PALM; STORM; SsrA; SsrB; photoactivatable fluorescent proteins; single molecule; super-resolution.

Publication types

  • Review

MeSH terms

  • Bacteria / ultrastructure*
  • Bacterial Secretion Systems / ultrastructure*
  • Bacteriological Techniques
  • Fluorescent Dyes / metabolism
  • Optical Imaging
  • Virulence Factors / metabolism

Substances

  • Bacterial Secretion Systems
  • Fluorescent Dyes
  • Virulence Factors