A complete set of monosomic alien addition lines developed from Gossypium anomalum in a Gossypium hirsutum background: genotypic and phenotypic characterization

Shan Meng; Zhenzhen Xu; Peng Xu; Aimin Chen; Qi Guo; Liang Zhao; Xianglong Chen; Tian Wen; Xianggui Zhang; Guowei Zhang; Wanchao Ni; Xinlian Shen

doi:10.1270/jsbbs.19146

A complete set of monosomic alien addition lines developed from Gossypium anomalum in a Gossypium hirsutum background: genotypic and phenotypic characterization

Breed Sci. 2020 Sep;70(4):494-501. doi: 10.1270/jsbbs.19146. Epub 2020 Jul 3.

Authors

Shan Meng^{1

2}, Zhenzhen Xu^{1

2}, Peng Xu^{1

2}, Aimin Chen³, Qi Guo^{1

2}, Liang Zhao^{1

2}, Xianglong Chen^{1

2}, Tian Wen³, Xianggui Zhang^{1

2}, Guowei Zhang^{1

2}, Wanchao Ni^{1

2}, Xinlian Shen^{1

2}

Affiliations

¹ Key Laboratory for Cotton and Canola Research at the Lower Reach of the Yangtze River Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China.
² Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences (JAAS), Nanjing 210014, China.
³ JOIN HOPE SEEDS Co., Ltd., Changji, Xinjiang 831100, China.

Abstract

Gossypium anomalum (B₁B₁) is a valuable wild resource for the genetic improvement of G. hirsutum (A₁A₁D₁D₁) in terms of fiber quality and disease and pest resistance, but the inherent difficulties in distant hybridization hinder its utilization in breeding programs. Monosomic alien addition lines (MAALs) are powerful tools for interspecific gene transfer. First, to access useful genes from G. anomalum, a fertile hexaploid from G. hirsutum × G. anomalum was obtained and then additional chromosomes were selected using SSR markers in successive backcrosses and self-crossing from BC₂F₁ to BC₄F₄. Finally, a complete set of 13 MAALs were developed. All the MAALs were confirmed by chromosome-specific anchored SSRs and genome-wide resequencing. The MAALs demonstrated abundant variation in morphological, agronomic, yield-related, and fiber quality traits. MAAL_3B had excellent fiber strength and fineness, indicating that the transmitted chromosome may carry desirable genes for the observed phenotypes. This complete set of MAALs will provide important genetic bridge material for the identification and introgression of favorable genes from G. anomalum and lay an important foundation for the genetic improvement of cotton.

Keywords: Gossypium anomalum; Gossypium hirsutum; complete set; genome resequencing; marker-assisted selection; monosomic alien addition line.