CRISPR-Cas12a trans-cleaves DNA G-quadruplexes

Ying Li; Tao Li; Bi-Feng Liu; Rui Hu; Jiang Zhu; Ting He; Xin Zhou; Conggang Li; Yunhuang Yang; Maili Liu

doi:10.1039/d0cc05540a

CRISPR-Cas12a trans-cleaves DNA G-quadruplexes

Chem Commun (Camb). 2020 Oct 20;56(83):12526-12529. doi: 10.1039/d0cc05540a.

Authors

Ying Li¹, Tao Li¹, Bi-Feng Liu², Rui Hu¹, Jiang Zhu³, Ting He³, Xin Zhou¹, Conggang Li¹, Yunhuang Yang¹, Maili Liu¹

Affiliations

¹ State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences - Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430071, China. liying@wipm.ac.cn ml.liu@wipm.ac.cn and University of Chinese Academy of Sciences, Beijing, 10049, China.
² The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
³ State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences - Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430071, China. liying@wipm.ac.cn ml.liu@wipm.ac.cn.

PMID: 32966423
DOI: 10.1039/d0cc05540a

Abstract

We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.

MeSH terms

Aptamers, Peptide / metabolism
Bacterial Proteins / chemistry
Bacterial Proteins / metabolism*
CRISPR-Associated Proteins / chemistry
CRISPR-Associated Proteins / metabolism*
CRISPR-Cas Systems / genetics
Circular Dichroism
DNA Cleavage
Endodeoxyribonucleases / chemistry
Endodeoxyribonucleases / metabolism*
Fluorescence Resonance Energy Transfer
Fluorescent Dyes / chemistry
G-Quadruplexes*
Humans
Kinetics
RNA, Guide, CRISPR-Cas Systems / metabolism*
Telomere / metabolism

Substances

Aptamers, Peptide
Bacterial Proteins
CRISPR-Associated Proteins
Fluorescent Dyes
RNA, Guide, CRISPR-Cas Systems
Cas12a protein
Endodeoxyribonucleases