Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers

Hui Qian; Yimei Jia; Michael J McCluskie

doi:10.1007/978-1-0716-0795-4_28

Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers

Methods Mol Biol. 2021:2183:499-511. doi: 10.1007/978-1-0716-0795-4_28.

Authors

Hui Qian¹, Yimei Jia², Michael J McCluskie³

Affiliations

¹ Nanotechnology Research Center, National Research Council Canada, Edmonton, AB, Canada.
² Human Health Therapeutics, National Research Council Canada, Ottawa, ON, Canada.
³ Human Health Therapeutics, National Research Council Canada, Ottawa, ON, Canada. michael.mccluskie@nrc-cnrc.gc.ca.

PMID: 32959263
DOI: 10.1007/978-1-0716-0795-4_28

Abstract

Cryogenic transmission electron microscopy (Cryo-TEM) enables visualizing the physicochemical structure of nanocarriers in solution. Here, we demonstrate the typical applications of Cryo-TEM in characterizing archaeosome-based vesicles as antigen carriers, including the morphology and size of vaccine carriers. Cryo-TEM tomography, incorporated with immunogold labeling for identifying and localizing the antigens, reveals the antigen distribution within archaeosomes in three dimensions (3D).

Keywords: Cryo-TEM; Electron Tomography; Immunogold labeling; Plunge freezing; Vaccine delivery carriers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cryoelectron Microscopy*
Drug Carriers*
Drug Delivery Systems*
Microscopy, Electron, Transmission* / methods
Software
Vaccines / administration & dosage*
Vaccines, Virus-Like Particle

Substances

Drug Carriers
Vaccines
Vaccines, Virus-Like Particle