Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes

Methods Mol Biol. 2021:2183:9-18. doi: 10.1007/978-1-0716-0795-4_2.

Abstract

The immunoglobulin capture assay (ICA) enables the enrichment for pathogen-specific plasmablasts from individuals with a confirmed adaptive immune response to vaccination or disseminated infection. Only single recombinant antigens have been used previously as probes in this ICA and it was unclear whether the method was applicable to complex probes such as whole bacterial cells. Here, we describe the enrichment of plasmablasts specific for polysaccharide and protein antigens of both Streptococcus pneumoniae and Neisseria meningitidis using whole formalin-fixed bacterial cells as probes. The modified ICA protocol described here allowed for a pathogen-specific hmAb cloning efficiency of >80%.

Keywords: Bacteria; Immunoglobulin capture assay; Pathogen-specific plasmablasts; Vaccine antigen discovery; Whole cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / immunology
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / isolation & purification*
  • Antibody Affinity
  • Antibody Formation / immunology
  • Antibody Specificity / immunology*
  • Antigens, Bacterial / immunology
  • Bacteria / immunology*
  • Host-Pathogen Interactions
  • Humans
  • Immunoassay / methods*
  • Immunoglobulin G / immunology
  • Molecular Probes*
  • Plasma Cells / immunology
  • Plasma Cells / metabolism
  • Streptococcus pneumoniae / immunology

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Immunoglobulin G
  • Molecular Probes