Comparison of dilute acid pretreatment of agave assisted by microwave versus ultrasound to enhance enzymatic hydrolysis

Abstract

A comparison between microwave and ultrasound irradiations in the agave pretreatment using dilute sulfuric acid as catalyst was assessed for the first time. Pretreatments were performed using a Taguchi Orthogonal Array L9 (3⁴) to improve the hemicellulose removal and the agave digestibility. The results showed that under optimal conditions, the hemicellulose removal was superior in the pretreatment assisted with microwave (77.5%) compared to ultrasound (28.2%). Enzymatic hydrolysis yield of agave pretreated with microwave (MW_OC) was 2-fold higher than agave pretreated with ultrasound (US_OC). The relatively mild conditions of pretreatment with MW_OC allowed to obtain a hydrolyzed free of inhibitors with a high glucose concentration (47.7 g/L) at low solids loading (10% w/v). However, these conditions did not have a significant effect over the agave pretreated with ultrasound. The pretreatment assisted with MW_OC allowed to reduce time and temperature of the process compared to pretreatment with conventional heating.

Keywords: Acid pretreatment; Agave biomass; Hemicellulose removal; Microwave vs ultrasound.