The Analysis of Gap Junctional Intercellular Communication Among Osteocytes in Chick Calvariae by Fluorescence Recovery After Photobleaching

Ziyi Wang; Yoshihito Ishihara; Hiroshi Kamioka

doi:10.1007/7651_2020_322

The Analysis of Gap Junctional Intercellular Communication Among Osteocytes in Chick Calvariae by Fluorescence Recovery After Photobleaching

Methods Mol Biol. 2021:2346:215-223. doi: 10.1007/7651_2020_322.

Authors

Ziyi Wang^{1

2}, Yoshihito Ishihara³, Hiroshi Kamioka¹

Affiliations

¹ Department of Orthodontics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
² Research Fellow of Japan Society for the Promotion of Science, Tokyo, Japan.
³ Department of Orthodontics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan. ishihara@md.okayama-u.ac.jp.

PMID: 32949375
DOI: 10.1007/7651_2020_322

Abstract

This chapter describes the use of fluorescence recovery after photobleaching (FRAP) for analyzing gap junctional intercellular communication (GJIC) among osteocytes in chick calvariae by confocal laser scanning microscope.

Keywords: Fluorescence recovery after photobleaching; Gap-junctional intercellular communication; One-phase exponential association equation; Osteocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Communication*
Chickens
Fluorescence Recovery After Photobleaching*
Gap Junctions / chemistry*
Microscopy, Confocal
Osteocytes / chemistry*
Osteocytes / cytology
Skull / chemistry*
Skull / cytology