Dataset on amelogenesis-related genes variants (ENAM and ENAM interacting genes) and on human leukocyte antigen alleles (DQ2 and DQ8) distribution in children with and without molar-incisor hypomineralisation (MIH)

Luka Hočevar; Jernej Kovač; Katarina Trebušak Podkrajšek; Saba Battelino; Alenka Pavlič

doi:10.1016/j.dib.2020.106224

Dataset on amelogenesis-related genes variants (ENAM and ENAM interacting genes) and on human leukocyte antigen alleles (DQ2 and DQ8) distribution in children with and without molar-incisor hypomineralisation (MIH)

Data Brief. 2020 Aug 25:32:106224. doi: 10.1016/j.dib.2020.106224. eCollection 2020 Oct.

Authors

Luka Hočevar¹, Jernej Kovač², Katarina Trebušak Podkrajšek^{2

3}, Saba Battelino^{4

5}, Alenka Pavlič^{1

6}

Affiliations

¹ Department of Paediatric and Preventive Dentistry, Faculty of Medicine, University of Ljubljana, Hrvatski trg 6, Ljubljana 1000, Slovenia.
² University Children's Hospital, University Medical Centre Ljubljana, Bohoričeva 20, Ljubljana, Slovenia.
³ Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, Ljubljana, Slovenia.
⁴ Department of Otorhinolaryngology and Cervicofacial Surgery, University Medical Centre Ljubljana, Zaloška 2, Ljubljana, Slovenia.
⁵ Department of Otorhinolaryngology, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, Ljubljana, Slovenia.
⁶ Department of Paediatric and Preventive Dentistry, University Medical Centre Ljubljana, Hrvatski trg 6, Ljubljana, Slovenia.

Abstract

All children, who were born in 2004 and had undergone surgical treatment for recurrent acute tonsillitis and/or acute otitis media at the ear, nose and throat clinic (ENT) between 2004 and 2010, were called on dental examination and blood sampling. Out of 441 invitees, 113 children and their parents/legal guardians agreed to participate. The following data from this group of subjects are presented: the presence of clinical signs of molar-incisor hypomineralisation (MIH), the distribution of human leukocyte antigen (HLA) alleles DQ2 and DQ8 and eight single nucleotide polymorphisms (SNPs) located in amelogenesis-related genes (rs3796704 in the ENAM gene, rs546778141 in the AMBN gene, rs2106416 in the AMELX gene, rs7660807 and rs35286445 in the AMTN gene, rs4870723 in the COL14A1 gene, rs2245803 in the MMP20 gene, and rs3828054 in the TUFT1 gene). Data on clinical signs of MIH were collected in accordance with the recommendation and on the proposed MIH clinical data recording sheet [1], and with appropriate preliminary training and calibration. Data on HLA DQ2 and DQ8 haplotypes and on SNPs of amelogenesis-related genes were obtained using DNA isolated from blood samples taken from subjects. The HLA DQ2 and DQ8 alleles were determined using the EliGene® Coeliac RT Kits (90,048-RT; Elisabeth Pharmacon spol. s.r.o., Brno-Židenice, Czech Republic) on a 7500 Fast RT-PCR System (Applied Biosystems, Waltham, MA, USA). The distributions of SNPs in the amelogenesis-related genes were determined using high resolution melting (HRM) using the Type-IT HRM Master Mix (Qiagen), TaqMan genotyping assays (ID: C__25766207_10; Thermo Fisher Scientific, Waltham, MA, USA) with the TaqMan Universal Master Mix II, or Sanger sequencing using sequencing master mix BigDye® Terminator v3.1 (Applied Biosystems) and ABI 3500 Genetic Analyser (Applied Biosystems). L. Hočevar, J. Kovač, K. Trebušak Podkrajšek, S. Battelino, A. Pavlič, 2020. The possible influence of genetic aetiological factors on molar-incisor hypomineralisation, Arch. Oral. Biol. 118, 104848. https://doi.org/10.1016/j.archoralbio.2020.104848.

Keywords: Aetiology; CADD, Combined annotation dependent depletion; ENT, ear, nose and throat clinic; FPM, First permanent molar; HLA, human leukocyte antigen; HRM, high resolution melting; Human leukocyte antigen; MIH, molar-incisor hypomineralisation; Molar-incisor hypomineralisation; PEB, Post-eruptive enamel breakdown; SNP, single nucleotide polymorphism; Single nucleotide polymorphism; The amelogenesis-related genes.