Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes

Abstract

Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CIC_H) and calves with naturally occurring bronchopneumonia (CIC_D) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CIC_D IgG, unlike CIC_H IgG. In both CIC_H and CIC_D, oleic acid-containing phospholipids predominated. In CIC_D, the content of oleic and vaccenic acid was higher than in CIC_H, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CIC_H and CIC_D; 1280 nm large particles were present only in CIC_D. The effect of CIC_H and CIC_D on mononuclear cells (MNC) and granulocytes was analysed in vitro. CIC_H and CIC_D, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CIC_D only. These results indicate that structural differences of CIC_H and CIC_D might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.

Keywords: Calves bronchopneumonia; IgG; Immune complexes; Leukocytes; Lipids.