Genome-wide identification and functional analysis of the horizontally transferred genes in Penicillium

Mingshuang Wang; Ruoxin Ruan

doi:10.1016/j.ygeno.2020.09.025

Genome-wide identification and functional analysis of the horizontally transferred genes in Penicillium

Genomics. 2020 Nov;112(6):5037-5043. doi: 10.1016/j.ygeno.2020.09.025. Epub 2020 Sep 14.

Authors

Mingshuang Wang¹, Ruoxin Ruan²

Affiliations

¹ College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
² Hangzhou Academy of Agricultural Sciences, Hangzhou, China. Electronic address: buffalo126@126.com.

PMID: 32941984
DOI: 10.1016/j.ygeno.2020.09.025

Abstract

Horizontal gene transfer (HGT) is the transmission of genetic material between different evolutionary lineages and is believed to be an important source of genomic innovation in fungi. In this study, we searched for prokaryotic-derived HGTs in 23 fully sequenced genomes using a comprehensive phylogenomic pipeline followed by manual curation. We found strong support for 60 HGT events comprising 190 genes putatively acquired from bacteria. HGT affected all Penicillium species to various degrees. Gene duplication events happened to 3 HGT genes after the transmission. Most HGT events include genes encoding a variety of enzymes, which are associated with sugar, amino acid, and lipid metabolism. Transcriptome data from 6 Penicillium species revealed that 33 of 35 HGT genes showed expression under the conditions tested and 16 genes were differentially expressed. Our results suggest an important role for inter-domain gene transfers in shaping the genome of Penicillium fungi.

Keywords: Expression; Genomic innovation; Horizontal gene transfer; Osmotrophy; Penicillium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression
Gene Transfer, Horizontal*
Genome, Fungal
Metabolic Networks and Pathways / genetics
Penicillium / genetics*
Penicillium / metabolism