Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition

Helena Canever; Pietro Salvatore Carollo; Romain Fleurisson; Philippe P Girard; Nicolas Borghi

doi:10.1007/978-1-0716-0779-4_22

Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition

Methods Mol Biol. 2021:2179:289-299. doi: 10.1007/978-1-0716-0779-4_22.

Authors

Helena Canever¹, Pietro Salvatore Carollo¹, Romain Fleurisson¹, Philippe P Girard^{1

2}, Nicolas Borghi³

Affiliations

¹ Institut Jacques Monod, Université de Paris, CNRS, Paris, France.
² Faculty of Basic and Biomedical Sciences, Université de Paris, Paris, France.
³ Institut Jacques Monod, Université de Paris, CNRS, Paris, France. nicolas.borghi@ijm.fr.

PMID: 32939728
DOI: 10.1007/978-1-0716-0779-4_22

Abstract

Molecular Tension Microscopy has been increasingly used in the last years to investigate mechanical forces acting in cells at the molecular scale. Here, we describe a protocol to image the tension of the junctional protein E-cadherin in cultured epithelial cells undergoing Epithelial-Mesenchymal Transition (EMT). We report how to prepare cells and induce EMT, and how to acquire, analyze, and quantitatively interpret FRET data.

Keywords: E-cadherin; EMT; FRET Biosensor; Mechanotransduction; Microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cadherins / metabolism*
Dogs
Epithelial-Mesenchymal Transition*
Fluorescence Resonance Energy Transfer / methods*
Madin Darby Canine Kidney Cells

Substances

Cadherins