LvCSN5 is involved in WSSV infection via interaction with wsv006

Rui-Feng Zou; Miao Cai; Qing-Hui Liu

doi:10.1016/j.dci.2020.103870

LvCSN5 is involved in WSSV infection via interaction with wsv006

Dev Comp Immunol. 2021 Jan:114:103870. doi: 10.1016/j.dci.2020.103870. Epub 2020 Sep 13.

Authors

Rui-Feng Zou¹, Miao Cai², Qing-Hui Liu³

Affiliations

¹ Key Laboratory of Maricultural Organism Disease Control Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
² Key Laboratory of Maricultural Organism Disease Control Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Shanghai Ocean University, Shanghai, 201306, China.
³ Key Laboratory of Maricultural Organism Disease Control Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, China. Electronic address: liuqh@ysfri.ac.cn.

PMID: 32937164
DOI: 10.1016/j.dci.2020.103870

Abstract

As an extremely virulent pathogen, white spot syndrome virus (WSSV) greatly threatens shrimp aquaculture worldwide. The interaction between virus and host is important for viral infection. In the present study, a yeast two-hybrid (Y2H) library was constructed to clarify the functions of wsv006, and the interaction between wsv006 and shrimp Litopenaeus vannamei (L. vannamei) was analyzed. Furthermore, we explored the role of the wsv006-interacting molecule L. vannamei COP9 constitutive photomorphogenic-like protein subunit 5 (LvCSN5) in WSSV infection. Y2H assay showed that wsv006 interacted with LvCSN5, and co-immunoprecipitation (Co-IP) assay confirmed such interaction. Multiple alignments of amino acid sequences with other species revealed that the LvCSN5 had high identity with Penaeusmonodon CSN5 (PmCSN5). LvCSN5 was mainly expressed in intestine, eye and hepatopancreas. In addition, the relative expression of LvCSN5 was significantly up-regulated both in intestine and hepatopancreas following the WSSV challenge. Besides, the relative expressions of IE1 and VP28, as well as the viral copy numbers were significantly increased in the LvCSN5-silenced shrimp. Our findings suggested that LvCSN5 was involved in WSSV infection by interacting with wsv006.

Keywords: CSN5; Interaction; Litopenaeus vannamei; WSSV; wsv006.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arthropod Proteins* / genetics
Arthropod Proteins* / metabolism
COP9 Signalosome Complex* / genetics
COP9 Signalosome Complex* / metabolism
DNA Virus Infections* / immunology
DNA Virus Infections* / metabolism
Hepatopancreas* / metabolism
Host-Pathogen Interactions
Immediate-Early Proteins / metabolism
Immunity, Innate
Intestines* / metabolism
Penaeidae* / immunology
Protein Binding
RNA, Small Interfering / genetics
Two-Hybrid System Techniques
Up-Regulation
Viral Envelope Proteins / metabolism
Viral Proteins* / genetics
Viral Proteins* / metabolism
Virus Replication
White spot syndrome virus 1* / physiology

Substances

Arthropod Proteins
COP9 Signalosome Complex
Immediate-Early Proteins
RNA, Small Interfering
Viral Envelope Proteins
Viral Proteins
VP28 protein, white spot syndrome virus
wsv006 protein, white spot syndrome virus