Backbone and side-chain chemical shift assignments of p50 subunit of NF-κB transcription factor

Amrinder Singh; H Jane Dyson

doi:10.1007/s12104-020-09978-z

Backbone and side-chain chemical shift assignments of p50 subunit of NF-κB transcription factor

Biomol NMR Assign. 2021 Apr;15(1):29-33. doi: 10.1007/s12104-020-09978-z. Epub 2020 Sep 16.

Authors

Amrinder Singh¹, H Jane Dyson²

Affiliations

¹ Department of Integrative Structural and Computational Biology, Scripps Research, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.
² Department of Integrative Structural and Computational Biology, Scripps Research, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. dyson@scripps.edu.

Abstract

Nuclear Factor κB (NF-κB) is a family of five related transcription factors that recognize a κB DNA element on the promoter and enhancer regions of target genes and modulate their expression. Here we report a complete set of ¹H, ¹³C, ¹⁵N backbone and side chain resonance assignments for the p50 DNA binding and dimerization domains of the p50 homodimer form of the NF-κB transcription factor. The chemical shift data constitute a first step towards understanding the mechanism of interaction of the p50 homodimer with DNA.

Keywords: Chemical shift; NF-κB; Resonance assignments; p50 DBD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

NF-kappa B
Nuclear Magnetic Resonance, Biomolecular*
Transcription Factors

Substances

NF-kappa B
Transcription Factors

Abstract

Publication types

MeSH terms

Substances

Grants and funding