Manganese-enhanced MRI (MEMRI) in breast and prostate cancers: Preliminary results exploring the potential role of calcium receptors

Gabriella Baio; Marina Fabbi; Michele Cilli; Francesca Rosa; Simona Boccardo; Francesca Valdora; Sandra Salvi; Luca Basso; Laura Emionite; Eliana Gianolio; Silvio Aime; Carlo Emanuele Neumaier

doi:10.1371/journal.pone.0224414

Manganese-enhanced MRI (MEMRI) in breast and prostate cancers: Preliminary results exploring the potential role of calcium receptors

PLoS One. 2020 Sep 15;15(9):e0224414. doi: 10.1371/journal.pone.0224414. eCollection 2020.

Authors

Gabriella Baio^{1

2}, Marina Fabbi³, Michele Cilli⁴, Francesca Rosa², Simona Boccardo⁵, Francesca Valdora^{2

6}, Sandra Salvi⁷, Luca Basso², Laura Emionite⁴, Eliana Gianolio⁸, Silvio Aime⁸, Carlo Emanuele Neumaier²

Affiliations

¹ NNUH-Norfolk and Norwich University Hospital and Norwich Medical School, University of East Anglia, Norwich, United Kingdom.
² Diagnostic Imaging and Senology Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
³ Biotherapies Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
⁴ Animal Facility, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
⁵ Lung Cancer Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
⁶ Department of Health Sciences, University of Genoa, Genoa, Italy.
⁷ Department of Pathology, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
⁸ Department of Molecular Biotechnologies and Health Sciences & Molecular Imaging Center, University of Torino, Torino, Italy.

Abstract

Procedures: To preliminary assess the relationship between Manganese Enhanced Magnetic Resonance Imaging (MEMRI) and the expression of calcium receptors in human prostate and breast cancer animal models.

Methods: NOD/SCID mice were inoculated with MDA-MB-231 breast cancer cells and prostate PC3 cancer cells to develop orthotopic or pseudometastatic cancer animal models. Mice were studied on a clinical 3T scanner by using a prototype birdcage coil before and after intravenous injection of MnCl2. Assessment of receptor's status was carried out after the MR images acquisition by immunohistochemistry on excised tumours.

Results: Manganese contrast enhancement in breast or prostate cancer animal models well correlated with CaSR expression (p<0.01), whereas TRPV6 expression levels appeared not relevant to the Mn uptake.

Conclusion: Our preliminary results suggest that MEMRI appears an efficient tool to characterize human breast and prostate cancer animal models in the presence of different expression level of calcium receptors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Breast Neoplasms / diagnostic imaging*
Breast Neoplasms / pathology
Calcium / metabolism
Cell Line, Tumor
Chlorides / administration & dosage*
Chlorides / pharmacokinetics
Contrast Media / administration & dosage*
Contrast Media / pharmacokinetics
Feasibility Studies
Female
Humans
Immunohistochemistry
Injections, Intravenous
Magnetic Resonance Imaging / methods*
Male
Manganese Compounds / administration & dosage*
Manganese Compounds / pharmacokinetics
Mice
Pilot Projects
Prostatic Neoplasms / diagnostic imaging*
Prostatic Neoplasms / pathology
Receptors, Calcium-Sensing / metabolism
TRPV Cation Channels / metabolism
Tissue Distribution
Xenograft Model Antitumor Assays

Substances

Chlorides
Contrast Media
Manganese Compounds
Receptors, Calcium-Sensing
TRPV Cation Channels
TRPV6 channel
manganese chloride
Calcium

Grants and funding

This study was supported by internal research funding assigned to the Diagnostic Imaging and Senology Unit, IRCCS Ospedale Policlinico San Martino, 16132 Genoa, Italy.