Abstract
Mass spectrometry and some other biophysical methods, have made substantial contributions to the studies on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human proteins interactions. The most interesting feature of SARS-CoV-2 seems to be the structure of its spike (S) protein and its interaction with the human cell receptor. Mass spectrometry of spike S protein revealed how the glycoforms are distributed across the S protein surface. X-ray crystallography and cryo-electron microscopy made huge impact on the studies on the S protein and ACE2 receptor protein interaction, by elucidating the three-dimensional structures of these proteins and their conformational changes. The findings of the most recent studies in the scope of SARS-CoV-2-Human protein-protein interactions are described here.
Keywords:
MS; SARS coronavirus; glycosylation; spike protein-ACE2 interaction; structural techniques.
MeSH terms
-
Amino Acid Sequence
-
Angiotensin-Converting Enzyme 2
-
Betacoronavirus / chemistry*
-
Betacoronavirus / pathogenicity
-
Binding Sites
-
COVID-19
-
Coronavirus Infections / epidemiology*
-
Coronavirus Infections / virology
-
Gene Expression
-
Host-Pathogen Interactions
-
Humans
-
Models, Molecular
-
Pandemics*
-
Peptidyl-Dipeptidase A / chemistry*
-
Peptidyl-Dipeptidase A / genetics
-
Peptidyl-Dipeptidase A / metabolism
-
Pneumonia, Viral / epidemiology*
-
Pneumonia, Viral / virology
-
Protein Binding
-
Protein Conformation, alpha-Helical
-
Protein Conformation, beta-Strand
-
Protein Interaction Domains and Motifs
-
Receptors, Virus / chemistry*
-
Receptors, Virus / genetics
-
Receptors, Virus / metabolism
-
SARS-CoV-2
-
Sequence Alignment
-
Severe Acute Respiratory Syndrome / epidemiology*
-
Severe Acute Respiratory Syndrome / virology
-
Severe acute respiratory syndrome-related coronavirus / chemistry
-
Severe acute respiratory syndrome-related coronavirus / pathogenicity
-
Spike Glycoprotein, Coronavirus / chemistry*
-
Spike Glycoprotein, Coronavirus / genetics
-
Spike Glycoprotein, Coronavirus / metabolism
Substances
-
Receptors, Virus
-
Spike Glycoprotein, Coronavirus
-
spike protein, SARS-CoV-2
-
Peptidyl-Dipeptidase A
-
ACE2 protein, human
-
Angiotensin-Converting Enzyme 2