Sulfur dioxide enhance drought tolerance of wheat seedlings through H2S signaling

Abstract

Drought is one of the most common factors that limit plant growth and productivity. Sulfur dioxide (SO₂) has recently been found to play a benefical role in protection of plants against environmental stress. In this study, we investigated the effect of SO₂ on the physiological and molecular response of wheat seedlings to drought stress. Pretreatment with 10 mg/m³ SO₂ significantly increased the survival rate and relative water content (RWC) of wheat seedlings under drought stress, indicating that pre-exposure to appropriate level of SO₂ could enhance drought tolerance of plants. These responses were related to the enhanced proline accumulation in the drought-treated wheat seedlings that induced by SO₂ pretreatment. Meanwhile, SO₂ pretreatment increased the activities of superoxide dismutase (SOD) and peroxidase (POD), and effectively reduced the content of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) in drought-treated wheat seedlings, suggesting SO₂ could alleviate drought-induced oxidative damage by enhancing antioxidant defense system in plants. Expression analysis of transcription factor genes also showed that SO₂ pretreatment decreased the expression of TaNAC69, but the expression of TaERF1 and TaMYB30 changed slightly and maintained at higher levels in wheat seedlings in response to drought stress. Furthermore, SO₂ pretreatment triggered marked accumulation of hydrogen sulfide (H₂S) in wheat seedlings under drought stress. When scavenged H₂S by spraying Hypotaurine (HT), the activities of antioxidant enzymes and the expression of transcription factor genes were decreased, and the content of H₂O₂ and MDA increased to the level of drought treatment alone, suggesting a regulatory role of SO₂-induced H₂S in plant adaptation to drought stress. Together, this study indicated that SO₂ enhanced drought tolerance of wheat seedlings through H₂S signaling, and provided new strategy for enhancing plant tolerance to drought stress.

Keywords: Antioxidant defense; Drought tolerance; Hydrogen sulfide; Sulfur dioxide; Wheat.