Aberrant Expression of NCAPG is Associated with Prognosis and Progression of Gastric Cancer

Ding-Ping Sun; Chih-Chan Lin; Shih-Ting Hung; Yi-Yu Kuang; You-Cheng Hseu; Chia-Lang Fang; Kai-Yuan Lin

doi:10.2147/CMAR.S248318

Aberrant Expression of NCAPG is Associated with Prognosis and Progression of Gastric Cancer

Cancer Manag Res. 2020 Aug 25:12:7837-7846. doi: 10.2147/CMAR.S248318. eCollection 2020.

Authors

Ding-Ping Sun^{1

2}, Chih-Chan Lin³, Shih-Ting Hung³, Yi-Yu Kuang³, You-Cheng Hseu^{4

5

6}, Chia-Lang Fang^{7

8}, Kai-Yuan Lin^{3

9}

Affiliations

¹ Department of Surgery, Chi Mei Medical Center, Tainan, Taiwan.
² Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
³ Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan.
⁴ Department of Cosmeceutics, China Medical University, Taichung, Taiwan.
⁵ Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
⁶ Chinese Medicine Research Center, China Medical University, Taichung, Taiwan.
⁷ Department of Pathology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
⁸ Department of Pathology, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan.
⁹ Department of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.

Abstract

Introduction: Gastric cancer (GC), one of the most prevalent malignancies, is the third-leading cause of cancer-related deaths globally. The aim of this study is to investigate the involvement of non-structural maintenance of chromosomes condensin I complex subunit G (NCAPG) in the prognosis of GC.

Methods: Western blotting and immunostaining were employed to measure the NCAPG level in gastric tissues and cells. Kaplan-Meier analysis was applied to analyze the prognostic value of NCAPG in GC. RNA interference was applied to investigate the influence of the NCAPG silencing on GC cell growth and spread.

Results: NCAPG overexpression was associated with several clinicopathologic characteristics, including nodal status (P = 0.0378), distant metastasis (P = 0.0088), staging (P = 0.0230), vascular invasion (P = 0.0012), and disease-free survival (P = 0.004). Kaplan-Meier analysis revealed that NCAPG overexpression was positively correlated to poor GC patients disease-free and overall survival (P = 0.004 and P < 0.001, respectively). Univariate Cox regression analysis showed that the overexpression of NCAPG was a prognostic biomarker of GC (P = 0.005). In cultured GC cells, the knockdown of NCAPG suppressed cell proliferation, migration and invasion. Meanwhile, further studies revealed that the NCAPG silencing induces the G0/G1 cell cycle arrest and accordingly represses cell division. Finally, Western blotting showed that NCPAG knockdown dysregulated cell cycle- and epithelial-mesenchymal transition-related molecules.

Conclusion: Overall, the results reveal that NCAPG overexpression is a candidate prognostic biomarker and potential therapeutic target in GC.

Keywords: NCAPG; gastric cancer; invasion; prognosis; proliferation.

Grants and funding

This work was supported by a grant from Chi Mei Medical Center (108CM-TMU-03). The sponsor had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.