Oligonucleotide-Directed Protein Threading Through a Rigid Nanopore

Methods Mol Biol. 2021:2186:135-144. doi: 10.1007/978-1-0716-0806-7_10.

Abstract

Nanopore technology enables the detection and analysis of single protein molecules. The technique measures the ionic current passing through a single pore inserted in an electrically insulating membrane. The translocation of the protein molecule through the pore causes a modulation of the ionic current. Analysis of the ionic current reveals the biophysics of co-translocational unfolding and may be used to infer the amino acid sequence and posttranslational modifications of the molecule.

Keywords: Co-translocational unfolding; Nanopore; Protein; Sequencing; Single-molecule.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophysiology / methods*
  • Hemolysin Proteins / chemistry*
  • Hemolysin Proteins / metabolism*
  • Ion Channels*
  • Nanopores*
  • Nanotechnology / methods*
  • Oligonucleotides / chemistry
  • Oligonucleotides / metabolism*
  • Protein Processing, Post-Translational
  • Protein Unfolding
  • Staphylococcus aureus / metabolism

Substances

  • Hemolysin Proteins
  • Ion Channels
  • Oligonucleotides