Design and Assembly of Membrane-Spanning DNA Nanopores

Kerstin Göpfrich; Alexander Ohmann; Ulrich F Keyser

doi:10.1007/978-1-0716-0806-7_4

Design and Assembly of Membrane-Spanning DNA Nanopores

Methods Mol Biol. 2021:2186:33-48. doi: 10.1007/978-1-0716-0806-7_4.

Authors

Kerstin Göpfrich¹, Alexander Ohmann², Ulrich F Keyser³

Affiliations

¹ Cavendish Laboratory, University of Cambridge, Cambridge, UK.
² Cavendish Laboratory, University of Cambridge, Cambridge, UK. ao391@cam.ac.uk.
³ Cavendish Laboratory, University of Cambridge, Cambridge, UK. ufk20@cam.ac.uk.

PMID: 32918728
DOI: 10.1007/978-1-0716-0806-7_4

Abstract

Versatile lipid membrane-inserting nanopores have been made by functionalizing DNA nanostructures with hydrophobic tags. Here, we outline design and considerations to obtain DNA nanopores with the desired dimensions and conductance properties. We further provide guidance on their reconstitution into lipid membranes.

Keywords: DNA nanotechnology; DNA origami; Ionic conductance; Lipid membrane; Nanopores; Synthetic ion-channels.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
Drug Design*
Hydrophobic and Hydrophilic Interactions
Lipid Bilayers / chemistry*
Membrane Lipids / chemistry*
Nanopores*
Nanostructures / chemistry*
Nanotechnology / methods*

Substances

Lipid Bilayers
Membrane Lipids
DNA