Irrigation water could be a direct source of pathogenic microorganisms that contaminates fresh produce and causes human diseases. In this study, we evaluated the prevalence of Salmonella spp. and its serotypes Typhimurium and Enteritidis, antimicrobial susceptibility and multidrug resistance (MDR) status, and seasonal variation, of Salmonella spp. in irrigation water of King Abdullah Canal (KAC) and Wadi Shueib (WS) in Jordan. The study results demonstrated that 32.2% (29/90) of irrigation water was contaminated with Salmonella spp. and the irrigation water of WS was found highly contaminated (62.1%) with Salmonella spp. compared to KAC (37.9%). In addition, higher prevalence of Salmonella were recorded in spring (33.3%) and summer (36.3%) seasons, compared to winter seasons. Among the contaminated irrigation water samples, prevalence of Salmonella Typhimurium serotype was found in (37.9%) (11/29) samples, while S. Enteritidis was absent. The most common antimicrobial resistance of Salmonella isolates was observed as tetracycline (11/29, 37.9%) and 6 (20.7%) Salmonella Typhimurium isolates were found multidrug resistance (MDR). Thus, this study results confirmed that the irrigation water was contaminated with pathogenic MDR Salmonella spp.