Thymol, cardamom and Lactobacillus plantarum nanoparticles as a functional candy with high protection against Streptococcus mutans and tooth decay

Nayyer Karimi; Vahid Jabbari; Aylar Nazemi; Khudaverdi Ganbarov; Nasrin Karimi; Asghar Tanomand; Samad Karimi; Amin Abbasi; Bahman Yousefi; Ehsaneh Khodadadi; Hossein Samadi Kafil

doi:10.1016/j.micpath.2020.104481

Thymol, cardamom and Lactobacillus plantarum nanoparticles as a functional candy with high protection against Streptococcus mutans and tooth decay

Microb Pathog. 2020 Nov:148:104481. doi: 10.1016/j.micpath.2020.104481. Epub 2020 Sep 8.

Authors

Affiliations

¹ Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: karimi.nayyer@yahoo.com.
² Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
³ Department of Microbiology, Baku State University, Baku, Azerbaijan.
⁴ Tabriz University of Applied Science and Technology, Tabriz, Iran.
⁵ Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.
⁶ Department of Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
⁷ Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁸ Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁹ Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Kafilhs@tbzmed.ac.ir.

PMID: 32916244
DOI: 10.1016/j.micpath.2020.104481

Abstract

Due to the increasing resistance of microorganisms against antibiotics, the use of natural bioactive substances for the prevention of pathogenic bacteria is considered in food products. In this study, thymol, cardamom essential oil, L. plantarum cell-free supernatant (ATCC 14917), and their nanoparticle candies prepared and inhibition activities against S. mutans (ATCC 25175), which is important in causing tooth decay, was investigated. Moisture content, pH, and sensory analyzes of candies measured. Also, SEM and FTIR of treated candy samples were performed. All examined bioactive substances and their nanoparticles showed an inhibitory effect against S. mutans with different minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The prepared candies had pH 5.5 represented a bactericidal effect against S. mutans. SEM and FTIR results approved the antibacterial effects of prepared candies. According to the results, all of the prepared candies significantly decreased S. mutans in saliva compared to the control candy and they are suitable agents for S. mutans growth-inhibiting. Also, cardamom essential oil candy showed the most general acceptance in a sensory analysis by panelists.

Keywords: Cardamon essential oil; L. plantarum cell-Free supernatant; S. mutans; Thymol.

MeSH terms

Candy
Elettaria*
Lactobacillus plantarum*
Microbial Sensitivity Tests
Nanoparticles*
Streptococcus mutans
Thymol

Substances

Thymol