UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers

Enzyme Microb Technol. 2020 Oct:140:109620. doi: 10.1016/j.enzmictec.2020.109620. Epub 2020 Jun 7.

Abstract

A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H2O2). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu3(PO4)2) as the organic and inorganic components, respectively. HRP/Cu3(PO4)2 hybrid nanoflowers (HRP-HNFs) showed linear H2O2 detection range from 100 nM to 100 μM, moreover, HRP-HNFs exhibited good selectivity for H2O2 detection due to the specificity of HRP. In addition, HRP-HNFs have good reusability, excellent color stability and long-term storage stability, which indicate HRP-HNFs are promising for catalysis and biomedical applications.

Keywords: Enzyme immobilization; Horseradish peroxidase; Hybrid nanomaterials; Hydrogen peroxide.

MeSH terms

  • Biosensing Techniques
  • Catalysis
  • Colorimetry
  • Copper / chemistry*
  • Enzyme Stability
  • Enzymes, Immobilized
  • Horseradish Peroxidase / chemistry*
  • Horseradish Peroxidase / metabolism
  • Hydrogen Peroxide / isolation & purification*
  • Hydrogen Peroxide / metabolism
  • Nanostructures / chemistry*
  • Phosphates / chemistry*

Substances

  • Enzymes, Immobilized
  • Phosphates
  • Copper
  • Hydrogen Peroxide
  • Horseradish Peroxidase