Enhanced CO2 fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex

Seung Kyou You; Young Jin Ko; Sang Kyu Shin; Dong-Hyeuk Hwang; Dae Hee Kang; Hyeon Min Park; Sung Ok Han

doi:10.1016/j.biortech.2020.124072

Enhanced CO₂ fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex

Bioresour Technol. 2020 Dec:318:124072. doi: 10.1016/j.biortech.2020.124072. Epub 2020 Sep 3.

Authors

Seung Kyou You¹, Young Jin Ko¹, Sang Kyu Shin¹, Dong-Hyeuk Hwang¹, Dae Hee Kang¹, Hyeon Min Park¹, Sung Ok Han²

Affiliations

¹ Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea.
² Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea. Electronic address: samhan@korea.ac.kr.

PMID: 32911368
DOI: 10.1016/j.biortech.2020.124072

Abstract

Photosynthesis of C. vulgaris shows slow growth and low lipid production due to the low solubility of CO₂, and it is thus necessary to increase the dissolved inorganic carbon source to solve this problem. In this study, carbonic anhydrase (CA) was fused with dockerin to form a CA complex by cohesion-dockerin interaction. The CA complex was displayed on the surface of C. vulgaris by a cellulose binding module. The CA complex increased activity and stability compared to those of a single enzyme. Additionally, C. vulgaris showed an average of 1.6-fold rapid growth during log phase through the influence of the CA complex. The bicarbonate produced by the CA complex increased the lipid production about 1.7-fold (23.3%), compared to 13.6% for the control group. The present results suggest that the CA complex successfully enhances the CO₂ fixation, which should be an essential study for 4th generation biofuels.

Keywords: CO(2) fixation; Carbonic anhydrase complex; Chlorella vulgaris; Lipid.

MeSH terms

Biofuels
Carbon Dioxide
Carbonic Anhydrases*
Chlorella vulgaris*
Lipids

Substances

Biofuels
Lipids
Carbon Dioxide
Carbonic Anhydrases