Combined Preparation of Human Olfactory Ensheathing Cells in the Therapy of Post-Traumatic Cysts of the Spinal Cord

A D Voronova; O V Stepanova; M P Valikhov; A V Chadin; A S Semkina; E K Karsuntseva; G A Fursa; I V Reshetov; V P Chekhonin

doi:10.1007/s10517-020-04925-3

Combined Preparation of Human Olfactory Ensheathing Cells in the Therapy of Post-Traumatic Cysts of the Spinal Cord

Bull Exp Biol Med. 2020 Aug;169(4):539-543. doi: 10.1007/s10517-020-04925-3. Epub 2020 Sep 10.

Authors

A D Voronova¹, O V Stepanova^{2

3}, M P Valikhov^{2

3}, A V Chadin², A S Semkina², E K Karsuntseva^{2

4}, G A Fursa^{2

4}, I V Reshetov⁵, V P Chekhonin²

Affiliations

¹ Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia. nastyanastyav@mail.ru.
² Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
³ Depatrment of Neurohumoral and Immunological Studies, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia.
⁴ M. V. Lomonosov Moscow State University, Moscow, Russia.
⁵ University Clinical Hospital No. 1, I. M. Se-chenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.

PMID: 32910386
DOI: 10.1007/s10517-020-04925-3

Abstract

In experiments on rats, co-transplantation of olfactory ensheathing cells of the human olfactory mucosa and neural stem/progenitor cells from the same source into post-traumatic cysts of the spinal cord led to improvement of the motor activity of the hind limbs and reduced the size of the cysts in some animals by 4-12%. The transplantation of a combination of the olfactory mucosa cells is effective and can be used in preclinical trials for the treatment of spinal cord injuries.

Keywords: cell therapy; human olfactory mucosa; neural stem/progenitor cells; olfactory ensheathing cells; post-traumatic cysts of spinal cord.

MeSH terms

Animals
Cell- and Tissue-Based Therapy / methods
Cysts / pathology
Cysts / physiopathology
Cysts / therapy*
Epithelial Cells / cytology
Epithelial Cells / physiology
Epithelial Cells / transplantation*
Female
Humans
Motor Activity / physiology
Neural Stem Cells / cytology
Neural Stem Cells / physiology
Neural Stem Cells / transplantation*
Olfactory Mucosa / cytology
Olfactory Mucosa / physiology
Primary Cell Culture
Rats
Rats, Wistar
Recovery of Function / physiology*
Spheroids, Cellular / cytology
Spheroids, Cellular / physiology
Spheroids, Cellular / transplantation*
Spinal Cord / pathology
Spinal Cord / physiopathology
Spinal Cord Injuries / pathology
Spinal Cord Injuries / physiopathology
Spinal Cord Injuries / therapy*
Transplantation, Heterologous
Treatment Outcome