Differential Serodiagnostics of Latent Stages of Syphilis Based on Measuring IgG and IgM Levels towards Extended Panel of Recombinant Antigens of T. pallidum

Bull Exp Biol Med. 2020 Aug;169(4):470-473. doi: 10.1007/s10517-020-04911-9. Epub 2020 Sep 10.

Abstract

Immunochips containing 12 recombinant antigens of T. pallidum (Тр15, Тр17, Тр47, TmpA, Тр0163, Тр0277, Тр0319, Тр0453, Тр0684, Тр0965, Тр0971, and Тр1038) were prepared to assay for IgG and IgM in serum samples (n=68) of healthy individuals and patients with the latent stages of syphilis. The linear discriminant analysis of detected IgG and IgM differentiated three groups of serum samples as 1) early latent syphilis; 2) seroresistant early latent syphilis; and 3) late latent syphilis with overall differentiation potency of 95.6% (88.9-100%). The samples of all syphilis patients were differentiated from the samples of healthy individuals with 100% specificity.

Keywords: T. pallidum; differential serodiagnostics; immunoarray; recombinant antigens; syphilis.

MeSH terms

  • Adult
  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / blood*
  • Antigens, Bacterial / classification
  • Case-Control Studies
  • Discriminant Analysis
  • Disease Progression
  • Female
  • Humans
  • Immunoassay*
  • Immunoglobulin G / blood*
  • Immunoglobulin M / blood*
  • Male
  • Middle Aged
  • Protein Array Analysis
  • Sensitivity and Specificity
  • Syphilis / blood
  • Syphilis / diagnosis*
  • Syphilis / immunology
  • Syphilis / microbiology
  • Treponema pallidum / immunology*
  • Treponema pallidum / pathogenicity

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Immunoglobulin G
  • Immunoglobulin M