O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B

Rossella Di Guida; Angela Casillo; Maria Michela Corsaro

doi:10.1016/j.carres.2020.108125

O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B

Carbohydr Res. 2020 Nov:497:108125. doi: 10.1016/j.carres.2020.108125. Epub 2020 Aug 17.

Authors

Rossella Di Guida¹, Angela Casillo², Maria Michela Corsaro³

Affiliations

¹ Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy. Electronic address: rossella.diguida@unina.it.
² Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy. Electronic address: angela.casillo@unina.it.
³ Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy. Electronic address: corsaro@unina.it.

PMID: 32905875
DOI: 10.1016/j.carres.2020.108125

Abstract

Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: →4)-α-l-GulpNAc3OAcAN-(1 →3)-β-d-QuipNAc-(1→ .

Keywords: Gulopyranuronamide; Lipopolysaccharide; NMR; Pseudomonas; Psychrotolerant.

MeSH terms

Carbohydrate Sequence
Hydrolysis
O Antigens / chemistry*
O Antigens / isolation & purification*
Pseudomonas / chemistry*
Solvents / chemistry

Substances

O Antigens
Solvents