Dataset on transcriptomic profiling of cholestatic liver injury in an in vitro and in vivo animal model

Eva Gijbels; Lindsey Devisscher; Mathieu Vinken

doi:10.1016/j.dib.2020.106156

Dataset on transcriptomic profiling of cholestatic liver injury in an in vitro and in vivo animal model

Data Brief. 2020 Aug 7:32:106156. doi: 10.1016/j.dib.2020.106156. eCollection 2020 Oct.

Authors

Eva Gijbels¹, Lindsey Devisscher², Mathieu Vinken¹

Affiliations

¹ Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
² Basic and Applied Medical Sciences, Gut-Liver Immunopharmacology Unit, Faculty of Medicine and Health Sciences, Ghent University, Corneel Heymanslaan 10, 9000 Ghent, Belgium.

Abstract

The transcriptomic dataset (whole genome microarray Affymetrix Human U133 plus 2.0 and Affymetrix Mouse Genome 430 2.0) presented in this paper describes the differential gene expression profile of a human in vitro model of drug-induced cholestasis and a well-known mouse in vivo model of cholestasis. The in vitro model consists of human hepatoma HepaRG cells in monolayer configuration exposed to 3 different cholestatic drugs with or without bile acids. For in vivo modelling of cholestasis, mice were subjected to bile duct ligation surgery. Consecutive normalization, summarization and background adjustments have been made by means of Robust Multichip Average Express software.

Keywords: Bile duct ligation; Drug-induced cholestasis; Human hepatoma HepaRG cells; Microarray; Transcriptomics.