Comparative assessment of the acute toxicity, haematological and genotoxic effects of ten commonly used pesticides on the African Catfish, Clarias gariepinus Burchell 1822

Nnamdi Henry Amaeze; Benjamin Olarewaju Komolafe; Abdulbashir Femi Salako; Kingsley Kelechi Akagha; Tam-Miete Dawari Briggs; Opeyemi Olayode Olatinwo; Micheal Abiodun Femi

doi:10.1016/j.heliyon.2020.e04768

Comparative assessment of the acute toxicity, haematological and genotoxic effects of ten commonly used pesticides on the African Catfish, Clarias gariepinus Burchell 1822

Heliyon. 2020 Aug 24;6(8):e04768. doi: 10.1016/j.heliyon.2020.e04768. eCollection 2020 Aug.

Authors

Nnamdi Henry Amaeze¹, Benjamin Olarewaju Komolafe¹, Abdulbashir Femi Salako¹, Kingsley Kelechi Akagha¹, Tam-Miete Dawari Briggs¹, Opeyemi Olayode Olatinwo¹, Micheal Abiodun Femi¹

Affiliation

¹ Ecotoxicology Laboratory, Environmental Toxicology and Pollution Management Unit, Zoology Department, University of Lagos, Akoka-Yaba, 101017 Lagos, Nigeria.

Abstract

Freshwater fishes are faced with increasing threats due to intensification of agriculture. This study evaluated the haematological and genotoxic effects of exposure of the African Catfish, Clarias gariepinus to sublethal concentrations of commonly used pesticides in agricultural settings. The evaluated pesticides were abamectin, carbofuran, chlorpyrifos, cypermethrin, deltamethrin, dichlorvos, dimethoate, fipronil, lambda-cyhalothrin and paraquat. The fishes were initially exposed singly to the pesticides for 96 h periods to determine their LC₅₀, followed by exposure to sublethal concentrations (1/100^th 96 h LC₅₀) over a 21 d period. In all cases, a control experiment with catfishes kept in dechlorinated municipal water was monitored simultaneously. The 96 h LC₅₀ values was found to vary widely from 2.043 μgL^-1 (Lambda-cyhalothrin) to 10284.288 μgL^-1(Paraquat). Significant differences (P < 0.05) were observed between mean haematological parameters-WBC, RBC, HGB, HCT, MCH and MCHC in the exposed and control catfishes. More so, micronucleus and nuclear abnormalities occurred at significantly higher proportions in pesticide exposed catfishes. Holistic cradle to grave approach as well as fate analysis is required to mitigate the potential harmful effects of pesticides to fresh water fishes.

Keywords: Agricultural science; Agrochemicals; Aquatic pollution; Mutagenesis; Oxidative stress.