Giant cell tumor stromal cells: osteoblast lineage-derived cells secrete IL-6 and IL-10 for M2 macrophages polarization

Kuan Yang; Lihui Bao; Xiaoning He; Wanmin Zhao; Dongdong Fei; Bei Li; Yang Xue; Zhiwei Dong

doi:10.7717/peerj.9748

Giant cell tumor stromal cells: osteoblast lineage-derived cells secrete IL-6 and IL-10 for M2 macrophages polarization

PeerJ. 2020 Aug 24:8:e9748. doi: 10.7717/peerj.9748. eCollection 2020.

Authors

Kuan Yang^#¹, Lihui Bao^#², Xiaoning He¹, Wanmin Zhao¹, Dongdong Fei¹, Bei Li^{1

2}, Yang Xue³, Zhiwei Dong¹

Affiliations

¹ State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
² Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an, Shaanxi, China.
³ State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.

^# Contributed equally.

Abstract

Background: The giant cell tumor (GCT) is a benign tumor which consists of three types cells: mononuclear histiocytic cells (MNHCs), multinuclear giant cells (MNGCs), and GCT stromal cells (GCTSCs). Numerous studies claim that GCTSCs have mesenchymal stem cells (MSCs) characters and play an important role in osteoclastogenesis; however, there are no research studies concerning macrophage polarization among GCT, which can be regarded as an ingredient for tumor aggression.

Method: We tested the effect of GCTSCs from three GCT samples which were collected from patients on proliferation, apoptosis and polarization of macrophage.

Result: In this article, we verified that GCTSCs expressed MSCs markers and had higher proliferation and relative lower differentiation abilities compared with BMMSCs. What's more, we found a higher proportion of M2 macrophages among neoplasm. Co-culturing GCTSCs with macrophages resulted in prominent macrophage M2 polarization and increased the release of IL-6 (Interleukin-6) and IL-10 (Interleukin-10)from GCTSCs. In conclusion, GCTSCs, as originating from MSCs, can secret IL-6 and IL-10, which may play a significant role in macrophage M2 polarization.

Keywords: Interleukin-6; Macrophages; Polarization; Giant cell tumor stromal cells; Interleukin-10.

Grants and funding

This work was supported by the grants from the National Key Research and Development Program of China (Nos. 2016YFC1101400), the National Natural Science Foundation of China (Nos. 81991504 and 81870768) and the Guangzhou Science, Technology and Innovation Commission (201803040008, 201704030024). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.