Abstract
A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Chromosome Deletion
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Cloning, Molecular
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Drug Resistance, Microbial / genetics
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Escherichia coli / drug effects
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Kanamycin / pharmacology
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Mutation
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Nucleic Acid Hybridization
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Plasmids
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Tetrahydrofolate Dehydrogenase / genetics*
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Tetrahydrofolate Dehydrogenase / isolation & purification
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Thymidylate Synthase / genetics
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Transduction, Genetic
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Transformation, Bacterial
Substances
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Kanamycin
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Tetrahydrofolate Dehydrogenase
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Thymidylate Synthase