Multicentric Analysis of the Species Distribution and Antifungal Susceptibility of Cryptic Isolates from Aspergillus Section Fumigati

S Imbert; A C Normand; S Cassaing; F Gabriel; L Kristensen; C Bonnal; L Lachaud; D Costa; J Guitard; L Hasseine; M Palous; M Piarroux; M Hendrickx; R Piarroux; A Fekkar

doi:10.1128/AAC.01374-20

Multicentric Analysis of the Species Distribution and Antifungal Susceptibility of Cryptic Isolates from Aspergillus Section Fumigati

Antimicrob Agents Chemother. 2020 Nov 17;64(12):e01374-20. doi: 10.1128/AAC.01374-20. Print 2020 Nov 17.

Authors

S Imbert^{1

2}, A C Normand³, S Cassaing⁴, F Gabriel⁵, L Kristensen⁶, C Bonnal⁷, L Lachaud⁸, D Costa⁹, J Guitard¹⁰, L Hasseine¹¹, M Palous³, M Piarroux^{3

12}, M Hendrickx¹³, R Piarroux^{3

12}, A Fekkar^{3

2}

Affiliations

¹ AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie-Mycologie, Paris, France sebastien.imbert@u-bordeaux.fr.
² Sorbonne Université, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, Paris, France.
³ AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie-Mycologie, Paris, France.
⁴ Centre Hospitalier Universitaire de Toulouse, Service de Parasitologie-Mycologie, Toulouse, France.
⁵ Centre Hospitalier Universitaire de Bordeaux, Service de Parasitologie-Mycologie, Bordeaux, France.
⁶ Aarhus University Hospital, Department of Clinical Microbiology, Aarhus, Denmark.
⁷ AP-HP, Hôpital Bichat-Claude Bernard, Service de Parasitologie-Mycologie, Paris, France.
⁸ Centre Hospitalier Universitaire de Montpellier, Service de Parasitologie-Mycologie, Montpellier, France.
⁹ Centre Hospitalier Universitaire de Rouen, Service de Parasitologie-Mycologie, Rouen, France.
¹⁰ AP-HP, Hôpital Saint-Antoine, Service de Parasitologie-Mycologie, Paris, France.
¹¹ Centre Hospitalier Universitaire de Nice, Service de Parasitologie-Mycologie, Nice, France.
¹² Sorbonne Université, INSERM, Institut Pierre Louis d'Epidemiologie et de Santé Publique, Paris, France.
¹³ Service of Mycology and Aerobiology, BCCM/IHEM Fungal Collection, Scientific Institute of Public Health, Brussels, Belgium.

Abstract

The antifungal susceptibility of Aspergillus cryptic species is poorly known. We assessed 51 isolates, belonging to seven Fumigati cryptic species, by the EUCAST reference method and the concentration gradient strip (CGS) method. Species-specific patterns were observed, with high MICs for azole drugs, except for Aspergillus hiratsukae and Aspergillus tsurutae, and high MICs for amphotericin B for Aspergillus lentulus and Aspergillus udagawae Essential and categorical agreements between EUCAST and CGS results were between 53.3 and 93.3%.

Keywords: Aspergillus lentulus; Aspergillus section Fumigati; Aspergillus thermomutatus; MALDI-TOF mass spectrometry; antifungal susceptibility testing; azole resistance; cryptic species.

Publication types

Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Antifungal Agents* / pharmacology
Aspergillus* / drug effects
Microbial Sensitivity Tests

Substances

Antifungal Agents

Supplementary concepts

Aspergillus hiratsukae
Aspergillus lentulus
Aspergillus tsurutae
Aspergillus udagawae