Abstract
There is currently a lack of efficient reagents to transfect cells with large plasmid DNA, which would be enabling tools for gene editing using CRISPR/Cas9 technology. Herein, we report the discovery of peptide dendrimer Z22 as a non-viral vector for transfecting large CRISPR/Cas9 pDNA into 3D-tumor spheroids with exceptionally high efficiency, low cytotoxicity and low immunogenicity.
MeSH terms
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CRISPR-Cas Systems
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Cell Survival / drug effects
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DNA / chemistry*
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DNA / metabolism
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Dendrimers / chemistry*
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Dendrimers / metabolism
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Gene Editing
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Genetic Therapy
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HEK293 Cells
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HeLa Cells
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Heparin / chemistry
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Humans
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Peptides / chemistry*
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Phosphatidylethanolamines / chemistry
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Piperidines / chemistry
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Spheroids, Cellular / chemistry
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Transfection / methods*
Substances
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Dendrimers
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Peptides
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Phosphatidylethanolamines
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Piperidines
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piperidine
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1,2-dielaidoylphosphatidylethanolamine
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Heparin
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DNA