Vaccine Based on Outer Membrane Vesicles Using Hydrogels as Vaccine Delivery System

Yadira Pastor; Isaiah Ting; Melibea Berzosa; Juan M Irache; Carlos Gamazo

doi:10.1007/978-1-0716-0791-6_14

Vaccine Based on Outer Membrane Vesicles Using Hydrogels as Vaccine Delivery System

Methods Mol Biol. 2021:2182:153-160. doi: 10.1007/978-1-0716-0791-6_14.

Authors

Yadira Pastor¹, Isaiah Ting¹, Melibea Berzosa¹, Juan M Irache², Carlos Gamazo³

Affiliations

¹ Department of Microbiology, University of Navarra, Pamplona, Spain.
² Department of Pharmacy and Pharmaceutical Technology, University of Navarra, Pamplona, Spain.
³ Department of Microbiology, University of Navarra, Pamplona, Spain. cgamazo@unav.es.

PMID: 32894494
DOI: 10.1007/978-1-0716-0791-6_14

Abstract

A simple procedure for obtaining outer membrane vesicles from Salmonella enterica and the use of hydrogels as vaccine delivery system is described. A heat treatment in saline solution of whole bacteria rendered the release of outer membrane vesicles containing relevant antigenic components. The immunogenicity of these antigens when administered by the intranasal route may be improved after embedment into hydrogels to increase residence half-time and thus activate the mucosal immune system.

Keywords: Acellular vaccine; Hydrogel; Outer membrane vesicle; Salmonella.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Intranasal / methods
Antigens, Bacterial / chemistry
Antigens, Bacterial / immunology
Bacterial Outer Membrane Proteins / chemistry*
Bacterial Outer Membrane Proteins / immunology*
Bacterial Proteins / chemistry
Bacterial Proteins / metabolism
Drug Delivery Systems / methods
Hydrogels / chemistry*
Immunity, Mucosal / immunology
Immunogenicity, Vaccine / immunology
Salmonella enterica / immunology
Vaccines / chemistry*
Vaccines / immunology*

Substances

Antigens, Bacterial
Bacterial Outer Membrane Proteins
Bacterial Proteins
Hydrogels
Vaccines