CRISPR Typing of Salmonella Isolates from Different Resources

Qiuchun Li

doi:10.1007/978-1-0716-0791-6_6

CRISPR Typing of Salmonella Isolates from Different Resources

Methods Mol Biol. 2021:2182:45-50. doi: 10.1007/978-1-0716-0791-6_6.

Author

Qiuchun Li¹

Affiliation

¹ Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China. qcli@yzu.edu.cn.

PMID: 32894486
DOI: 10.1007/978-1-0716-0791-6_6

Abstract

CRISPR typing is a newly developed method used to reveal the genetic relationship of bacterial isolates from different resources. For Salmonella, CRISPR typing can not only reveal the phylogenic difference among isolates belonging to the identical serotype, but also show good correspondence with Salmonella serotypes. Here we describe the protocol of CRISPR typing method used in Salmonella, and the approaches to analyze the genetic relationship among different strains.

Keywords: BioNumerics 7.5; CRISPR typing; Cluster analysis; Spacer.

MeSH terms

Bacterial Typing Techniques / methods*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Phylogeny
Salmonella / genetics*
Serogroup