Detection of Salmonella by the 3M Molecular Detection Assays: MDS® Method

Greici Bergamo; Eliezer Avila Gandra

doi:10.1007/978-1-0716-0791-6_4

Detection of Salmonella by the 3M Molecular Detection Assays: MDS^® Method

Methods Mol Biol. 2021:2182:33-38. doi: 10.1007/978-1-0716-0791-6_4.

Authors

Greici Bergamo¹, Eliezer Avila Gandra²

Affiliations

¹ Laboratory of Food Science and Molecular Biology (LACABIM), Center for Chemical, Pharmaceutical and Food Sciences (CCQFA), Room 53, Building 04 - Capão do Leão Campus, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil. greici.bergamo@hotmail.com.
² Laboratory of Food Science and Molecular Biology (LACABIM), Center for Chemical, Pharmaceutical and Food Sciences (CCQFA), Room 53, Building 04 - Capão do Leão Campus, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil.

PMID: 32894484
DOI: 10.1007/978-1-0716-0791-6_4

Abstract

Developed by 3M Company, 3M ™ Molecular Detection Assays-3M MDS-enable detection of Salmonella from advanced isothermal DNA amplification and bioluminescence detection technology. It can be used for a wide variety of products, including poultry, eggs, pet foods, and environmental samples, and results are obtained within about 24 h. In this chapter, all steps of the 3M MDS™ method for detection of Salmonella are described and detailed.

Keywords: 3M MDA; 3M MDS; Bioluminescence detection; Isothermal DNA amplification; Pathogenic microorganisms.

MeSH terms

Animals
Bacteriological Techniques / methods*
Eggs / microbiology
Food Contamination / analysis
Food Microbiology / methods*
Nucleic Acid Amplification Techniques / methods*
Poultry / microbiology
Salmonella / genetics*