Diversity of L1/L2 genes and molecular epidemiology of high-level carbapenem resistance Stenotrophomonas maltophilia isolates from animal production environment in China

Lu Han; Rong-Min Zhang; Ling Jia; Shuan-Cheng Bai; Xu-Wei Liu; Ran Wei; Chao-Yue Cui; Ruan-Yang Sun; Min-Ge Wang; Da-Tong Cai; Xiao-Ping Liao; Ya-Hong Liu; Jian Sun

doi:10.1016/j.meegid.2020.104531

Diversity of L1/L2 genes and molecular epidemiology of high-level carbapenem resistance Stenotrophomonas maltophilia isolates from animal production environment in China

Infect Genet Evol. 2020 Dec:86:104531. doi: 10.1016/j.meegid.2020.104531. Epub 2020 Sep 4.

Authors

Affiliations

¹ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.
² National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.
³ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. Electronic address: jiansun@scau.edu.cn.

PMID: 32891878
DOI: 10.1016/j.meegid.2020.104531

Abstract

Stenotrophomonas maltophilia is emerging as a significant cause of human and animal disease worldwide. A total of 3400 samples were collected from animal farms and adjacent environments in China. The bla_L1 and bla_L2 genes were identified using whole genome sequence analyses and examined by phylogenetics. Isolates were also tested for susceptibility to 18 antibiotics. We isolated 118 strains of S. maltophilia from 3400 samples. The positive rates of bla_L1 and bla_L2 genes were 75% (89/118) and 22% (26/118) and we identified 11 L1 and 6 L2 amino acid sequence variants. S. maltophilia has at least two inducible β-lactamases (L1 and L2) that can hydrolyze almost all classes of β-lactams and these genes are suspected to confer carbapenem resistance. This represents a significant public health threat especially for hospitalized patients. We conducted a molecular surveillance study on the prevalence and characteristics of the bla_L1 and bla_L2 genes of S. maltophilia.

Keywords: Antibiotic resistance; Carbapenemase; L1 and L2; Stenotrophomonas maltophilia; β-Lactamases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Diseases / drug therapy
Animal Diseases / epidemiology*
Animal Diseases / microbiology*
Animals
Anti-Bacterial Agents / pharmacology
China / epidemiology
Gram-Negative Bacterial Infections / veterinary*
Microbial Sensitivity Tests
Molecular Epidemiology
Stenotrophomonas maltophilia / drug effects*
Stenotrophomonas maltophilia / genetics*
Stenotrophomonas maltophilia / isolation & purification
beta-Lactam Resistance*
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
beta-lactamase L1
beta-lactamase L2
beta-Lactamases