Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA

J Virol Methods. 2020 Dec:286:113965. doi: 10.1016/j.jviromet.2020.113965. Epub 2020 Sep 3.

Abstract

Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capacities delivering results in a rapid and reliable manner are a prerequisite. Here, we provide and validate such a rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA, termed COVID-quick-DET. This straightforward method operates with simple proteinase K treatment and repetitive heating steps with a sensitivity of 94.6% in head-to-head comparisons with kit-based isolation methods. This result is supported by data obtained from serially diluted SARS-CoV-2 virus stocks. Given its cost- and time-effective operation, COVID-quick-DET might be best suited for countries with general shortage or temporary acute scarcity of resources and equipment.

Keywords: COVID-19; Heating; Kit-free RNA extraction; Proteinase K; SARS-CoV-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Betacoronavirus / genetics*
  • Betacoronavirus / isolation & purification*
  • COVID-19
  • COVID-19 Testing
  • Clinical Laboratory Techniques / methods*
  • Coronavirus Infections / diagnosis*
  • Coronavirus Infections / virology*
  • Diagnostic Tests, Routine
  • Endopeptidase K / chemistry
  • Heating
  • Humans
  • Pandemics
  • Pneumonia, Viral / diagnosis*
  • Pneumonia, Viral / virology*
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification*
  • Reagent Kits, Diagnostic*
  • SARS-CoV-2
  • Sensitivity and Specificity

Substances

  • RNA, Viral
  • Reagent Kits, Diagnostic
  • Endopeptidase K