A test potential booster for fast-scan cyclic voltammetry with an electrophysiological amplifier

Anal Biochem. 2020 Dec 1:610:113934. doi: 10.1016/j.ab.2020.113934. Epub 2020 Sep 3.

Abstract

Fast-scan cyclic voltammetry (FSCV) is a powerful technique for studying the local dynamics of neurotransmitters and neuromodulators. FSCV is attractive to researchers employing electrophysiological techniques because it can be performed using an electrophysiological voltage-clamp amplifier. However, the narrow test potential range of electrophysiological amplifiers (typically, ±1 V) limits testable species of analytes. Here we devised a booster that extends the test potential range. Using the booster, we could detect the oxidation current of adenosine peaking near a test potential of +1.5 V. The booster should promote combined electrophysiological and electrochemical studies of synaptic release and neural secretion.

Keywords: Amperometry; Cyclic voltammetry; Electrochemistry; Electrophysiology; Voltage-clamp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / chemistry
  • Dopamine / analysis
  • Dopamine / chemistry
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Microelectrodes
  • Neurotransmitter Agents / analysis*
  • Neurotransmitter Agents / chemistry
  • Oxidation-Reduction

Substances

  • Neurotransmitter Agents
  • Adenosine
  • Dopamine