Comparative eye and liver differentially expressed genes reveal monochromatic vision and cancer resistance in the shortfin mako shark (Isurus oxyrinchus)

Rodrigo R Domingues; Vito Antonio Mastrochirico-Filho; Natalia J Mendes; Diogo T Hashimoto; Rui Coelho; Vanessa Paes da Cruz; Agostinho Antunes; Fausto Foresti; Fernando F Mendonça

doi:10.1016/j.ygeno.2020.08.037

Comparative eye and liver differentially expressed genes reveal monochromatic vision and cancer resistance in the shortfin mako shark (Isurus oxyrinchus)

Genomics. 2020 Nov;112(6):4817-4826. doi: 10.1016/j.ygeno.2020.08.037. Epub 2020 Sep 2.

Authors

Rodrigo R Domingues¹, Vito Antonio Mastrochirico-Filho², Natalia J Mendes², Diogo T Hashimoto², Rui Coelho³, Vanessa Paes da Cruz⁴, Agostinho Antunes⁵, Fausto Foresti⁴, Fernando F Mendonça⁶

Affiliations

¹ Departamento de Ciências do Mar, Instituto do Mar, Universidade Federal de São Paulo - UNIFESP, Rua Carvalho de Mendonça, 144, Santos, São Paulo 11070-100, Brazil; CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos, s/n, 4450-208 Porto, Portugal; Departmento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal. Electronic address: domingues.pesca@gmail.com.
² Centro de Aquicultura da UNESP, Universidade Estadual Paulista - UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, Jaboticabal, São Paulo 14884-900, Brazil.
³ Instituto Português do Mar e da Atmosfera (IPMA, I.P.), Av. 5 Outubro, 8700-305 Olhão, Portugal; Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
⁴ Laboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, Universidade Estadual Paulista - UNESP, Botucatu, São Paulo 18618-689, Brazil.
⁵ CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos, s/n, 4450-208 Porto, Portugal; Departmento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.
⁶ Departamento de Ciências do Mar, Instituto do Mar, Universidade Federal de São Paulo - UNIFESP, Rua Carvalho de Mendonça, 144, Santos, São Paulo 11070-100, Brazil.

PMID: 32890699
DOI: 10.1016/j.ygeno.2020.08.037

Abstract

The shortfin mako, Isurus oxyrinchus is an oceanic pelagic shark found worldwide in tropical and subtropical waters. However, the understanding of its biology at molecular level is still incipient. We sequenced the messenger RNA isolated from eye and liver tissues. De novo transcriptome yielded a total of 705,940 transcripts. A total of 3774 genes were differentially expressed (DEGs), with 1612 in the eye and 2162 in the liver. Most DEGs in the eye were related to structural and signaling functions, including nonocular and ocular opsin genes, whereas nine out of ten most overexpressed genes in the liver were related to tumor suppression, wound healing, and human diseases. Furthermore, DEGs findings provide insights on the monochromatic shark vision and a repertory of cancer-related genes, which may be insightful to elucidate shark resistance to cancer. Therefore, our results provide valuable sequence resources for future functional and population studies.

Keywords: Elasmobranch; Gene ontology; Photoreception; Transcriptomics; Tumor suppression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Resistance / genetics*
Eye
Eye Proteins / genetics*
Gene Expression
Liver*
Molecular Sequence Annotation
Neoplasms / genetics*
Opsins / genetics
RNA, Messenger / genetics
Sharks / genetics*
Transcriptome
Vision, Ocular / genetics

Substances

Eye Proteins
Opsins
RNA, Messenger