Macroautophagy, by its very nature, is a protein trafficking process. Cargos are transported and processed. Atg proteins come and go. In this chapter, we present three assays to monitor these dynamic events: a non-radioactive pulse-chase labeling assay to monitor the transport of prApe1 and two fluorescent microscopy-based assays to assess the trafficking of Atg8 and Atg9.
Keywords: Ape1; Atg8; Atg9; Autophagy; Cvt pathway; Fluorescent microscopy; Intracellular trafficking; Pulse-chase; Yeast.