The chemical structure of polysaccharides isolated from the Ochrobactrum rhizosphaerae PR17T

Nikola Szpakowska; Agnieszka Kowalczyk; Sylwia Jafra; Zbigniew Kaczyński

doi:10.1016/j.carres.2020.108136

The chemical structure of polysaccharides isolated from the Ochrobactrum rhizosphaerae PR17^T

Carbohydr Res. 2020 Nov:497:108136. doi: 10.1016/j.carres.2020.108136. Epub 2020 Aug 18.

Authors

Nikola Szpakowska¹, Agnieszka Kowalczyk¹, Sylwia Jafra², Zbigniew Kaczyński³

Affiliations

¹ Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland.
² Laboratory of Biological Plant Protection, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, University of Gdansk, A. Abrahama 58, 80-307, Gdansk, Poland.
³ Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland. Electronic address: zbigniew.kaczynski@ug.edu.pl.

PMID: 32889436
DOI: 10.1016/j.carres.2020.108136

Abstract

The bacteria from Ochrobactrum genus are commonly found in the soil and association with the roots of plants. The O-polysaccharide and the glucan were isolated from the Ochrobactrum rhizosphaerae PR17^T strain. Purified polysaccharides were analysed using chemical methods and NMR spectroscopy. Sugar and absolute configuration assignment combined with NMR data revealed the chemical structure of the repeating unit of the O.

Keywords: Glucan; NMR; O-polysaccharide; Ochrobactrum rhizosphaerae PR17(T); Structure.

MeSH terms

Carbohydrate Conformation
Models, Molecular
O Antigens / chemistry*
Ochrobactrum / chemistry*

Substances

O Antigens

Supplementary concepts

Ochrobactrum rhizosphaerae