Metabolic adjustment of Pyrrhulina aff. brevis exposed to different climate change scenarios

Abstract

The increases in CO₂ concentrations and, consequently, temperature due to climate change are predicted to intensify. Understanding the physiological responses of Pyrrhulina aff. brevis to the climatic scenarios proposed by the IPCC (2014) for the next 100 years is of fundamental importance to determine its susceptibility. Thus, the present study aimed to evaluate the effects of the predicted climatic scenarios for the year 2100 on the metabolic adjustments of P. aff. brevis . Specifically, the rate of oxygen uptake, electron transport system capacity, glycogen and lactate content and the role of Na⁺K⁺-ATPases and H⁺-ATPase were evaluated. P. aff. brevis individuals were exposed for 15 days to the simulated climatic scenarios in climate scenario rooms, where temperature and CO₂ in the air were controlled. Two rooms were used to simulate the climatic scenarios predicted by the IPCC (2014): moderate (RCP 6; 2.5 °C and 400 μatm CO₂ above current levels) and extreme (RCP 8.5; 4.5 °C and 900 μatm CO₂ above current levels), in addition to the "control room" that represents the current scenario. There was an increase in the metabolic rate (MO₂) in the animals acclimated to the climate change scenarios (RCP 6 and RCP 8.5) compared to the current scenario. These responses showed a typical effect of temperature on energy demand in relation to the increase in temperature and CO₂. Our data showed an increase in O₂ consumption (MO₂), lactate levels and H⁺-ATPase activity of the animals acclimated to the moderate and extreme climate change scenarios. Such adjustments presented a clear metabolic imbalance, an alteration that may imply challenges for survival, growth, distribution and reproduction in the face of the expected environmental changes for the year 2100.

Keywords: Adaptive mechanisms; Anaerobic metabolism; Central Amazon; Climate change.