Amplification-free profiling of microRNA-122 biomarker in DILI patient serums, using the luminex MAGPIX system

Antonio Marín-Romero; Mavys Tabraue-Chávez; James W Dear; Rosario M Sánchez-Martín; Hugh Ilyine; Juan J Guardia-Monteagudo; Mario A Fara; Francisco J López-Delgado; Juan J Díaz-Mochón; Salvatore Pernagallo

doi:10.1016/j.talanta.2020.121265

Amplification-free profiling of microRNA-122 biomarker in DILI patient serums, using the luminex MAGPIX system

Talanta. 2020 Nov 1:219:121265. doi: 10.1016/j.talanta.2020.121265. Epub 2020 Jun 29.

Affiliations

¹ DESTINA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada, 18100, Spain; GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada, Andalusian Regional Government, PTS Granada - Avenida de la Ilustración, 114 - 18016, GRANADA, Spain; Universidad de Granada. Facultad de Farmacia. Departamento de Quimica Farmacéutica y Orgánica, Campus Cartuja s/n, 18071, Granada, Spain.
² DESTINA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada, 18100, Spain.
³ Pharmacology,Therapeutics and Toxicology, Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute, 47, Little France Crescent, Edinburgh, EH16, 4TJ, UK.
⁴ GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada, Andalusian Regional Government, PTS Granada - Avenida de la Ilustración, 114 - 18016, GRANADA, Spain; Universidad de Granada. Facultad de Farmacia. Departamento de Quimica Farmacéutica y Orgánica, Campus Cartuja s/n, 18071, Granada, Spain.
⁵ DESTINA Genomics Ltd, 7-11 Melville St, Edinburgh, EH3 7PE, UK.
⁶ GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada, Andalusian Regional Government, PTS Granada - Avenida de la Ilustración, 114 - 18016, GRANADA, Spain; Universidad de Granada. Facultad de Farmacia. Departamento de Quimica Farmacéutica y Orgánica, Campus Cartuja s/n, 18071, Granada, Spain. Electronic address: juan@destinagenomics.com.
⁷ DESTINA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada, 18100, Spain. Electronic address: salvatore@destinagenomics.com.

PMID: 32887156
DOI: 10.1016/j.talanta.2020.121265

Abstract

Dynamic chemical labelling is a single-base specific method to enable detection and quantification of micro-Ribonucleic Acids in biological fluids without extraction and pre-amplification. In this study, dynamic chemical labelling was combined with the Luminex MAGPIX system to profile levels of microRNA-122 biomarker in serum from patients with Drug-Induced Liver Injury.

Keywords: Drug-induced liver injury (DILI); Dynamic chemical labelling (DCL); Liquid biopsy; Luminex MAGPIX; miRNA-122.

MeSH terms

Biomarkers
Chemical and Drug Induced Liver Injury*
Humans
MicroRNAs*

Substances

Biomarkers
MIRN122 microRNA, human
MicroRNAs