Reliable performance of aggregation-induced emission nanoparticle-based lateral flow assay for norfloxacin detection in nine types of animal-derived food

Abstract

The wide application of lateral flow assay (LFA) was limited by its low sensitivity and poor matric tolerance. Aggregation induced emission (AIE) materials show superior luminescence and good stability under close packing state, which may accelerate the development of LFA. However, the detection performance of the AIE-based LFA in different real samples was unclear. In this work, an AIE-LFA was established for norfloxacin (NOR) detection in nine types of animal-derived food. Results indicated that AIE-LFA had the average recovery range of 75.6%-95.1%, 78.6%-94.6%, 71.4%-112.7%, 81.7%-121.8%, 72.7%-93.5%, 79.8%-108.5%, 79.2%-109.4%, 76.3%-103.6%, and 80.6%-108.3% in pork, pig liver, fish, lamb, beef, milk, chicken, egg, and honey, respectively. The detection results of AIE-LFA were compatible with HPLC-MS/MS in detecting NOR in 135 real samples from nine types of animal-derived food. The developed AIE-LFA was sensitive and reliable for NOR detection in those real samples.

Keywords: Aggregation-induced emission; Animal-derived food; Lateral flow assay; Norfloxacin.