Preparation of NiMn2O4/C necklace-like microspheres as oxidase mimetic for colorimetric determination of ascorbic acid

Yu Ding; Minggang Zhao; Jiatuo Yu; Zhengming Li; Xiaomin Zhang; Ye Ma; Hui Li; Shougang Chen

doi:10.1016/j.talanta.2020.121299

Preparation of NiMn₂O₄/C necklace-like microspheres as oxidase mimetic for colorimetric determination of ascorbic acid

Talanta. 2020 Nov 1:219:121299. doi: 10.1016/j.talanta.2020.121299. Epub 2020 Jun 26.

Authors

Yu Ding¹, Minggang Zhao², Jiatuo Yu¹, Zhengming Li¹, Xiaomin Zhang¹, Ye Ma¹, Hui Li³, Shougang Chen¹

Affiliations

¹ Department of Materials Science and Engineering, Ocean University of China, 266100, Qingdao, PR China.
² Department of Materials Science and Engineering, Ocean University of China, 266100, Qingdao, PR China. Electronic address: zhaomg@ouc.edu.cn.
³ Optoelectronic Materials and Technologies Engineering Laboratory of Shandong, Physics Department,Qingdao University of Science and Technology,Qingdao, 266100,PR China.

PMID: 32887041
DOI: 10.1016/j.talanta.2020.121299

Abstract

The NiMn₂O₄/C necklace-like microspheres (NLM) were successfully prepared by hydrothermal method and oil bath. This unique necklace-like structure makes them exhibit the enhanced intrinsic oxidase-like activity, as the special interface can help capture electrons from 3,3',5,5'-tetramethylbenzidine. The fabricated NiMn₂O₄/C NLM were successfully used as the high-performance oxidase mimetic to catalyze the oxidation of TMB directly for the color reaction. A simple colorimetric method for detection of ascorbic acid by fading was developed, and the high sensitivity with the low detection limit (0.047 μM) was achieved. It is a facile route to fabricate the NiMn₂O₄/C NLM as the high-performance oxidase mimetic for colorimetric biosensing.

Keywords: Ascorbic acid; Biosensing; Colorimetric; NiMn(2)O(4)/C; Oxidase mimetic.