Electrochemical synthesis of Prussian blue from iron impurities in 3D-printed graphene electrodes: Amperometric sensing platform for hydrogen peroxide

Abstract

This communication shows the electrochemical synthesis of Prussian blue (PB) films on additive manufactured (3D-printed) electrodes from iron impurities found at the graphene-polylactic acid (G/PLA) substrate and its application as a highly selective sensor for H₂O₂. The 3D-printed G/PLA electrode was immersed in dimethylformamide for 30 min to exposure the iron impurities within the PLA matrix. Next, cyclic voltammograms (200 cycles) in the presence of potassium ferricyanide in 0.1 mol L^-1 KCl + 0.01 mol L^-1 HCl were performed to grow the PB films. The sensing properties of this novel PB/G/PLA platform were evaluated for the amperometric detection of H₂O₂ using batch-injection analysis, with a limit of detection of 0.56 μmol L^-1 under the application of 0.0 V (vs Ag/AgCl/KCl_sat.). The applicability of the sensor was demonstrated for the analysis of milk samples (10-fold diluted in the supporting electrolyte), resulting in proper recovery values (94-101%).

Keywords: Additive manufacture; Artificial peroxidase; Batch-injection analysis; Disposable electrochemical sensors; Iron hexacyanoferrate.