Genotype-phenotype correlation of β-lactamase-producing uropathogenic Escherichia coli (UPEC) strains from Bangladesh

Maqsud Hossain; Tahmina Tabassum; Aura Rahman; Arman Hossain; Tamanna Afroze; Abdul Mueed Ibne Momen; Abdus Sadique; Mrinmoy Sarker; Fariza Shams; Ahmed Ishtiaque; Abdul Khaleque; Munirul Alam; Anwar Huq; Gias U Ahsan; Rita R Colwell

doi:10.1038/s41598-020-71213-5

Genotype-phenotype correlation of β-lactamase-producing uropathogenic Escherichia coli (UPEC) strains from Bangladesh

Sci Rep. 2020 Sep 3;10(1):14549. doi: 10.1038/s41598-020-71213-5.

Authors

Maqsud Hossain^{1

2}, Tahmina Tabassum^#^{1

2}, Aura Rahman^#^{1

2}, Arman Hossain^{1

2}, Tamanna Afroze¹, Abdul Mueed Ibne Momen^{1

2}, Abdus Sadique¹, Mrinmoy Sarker², Fariza Shams², Ahmed Ishtiaque², Abdul Khaleque², Munirul Alam³, Anwar Huq⁴, Gias U Ahsan^{1

5}, Rita R Colwell^{6

7

8}

Affiliations

¹ NSU Genome Research Institute (NGRI), North South University, Dhaka, Bangladesh.
² Department of Biochemistry and Microbiology, North South University, Dhaka, Bangladesh.
³ International Centre for Diarrheal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
⁴ Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA.
⁵ Department of Public Health, North South University, Dhaka, Bangladesh.
⁶ Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA. rcolwell@umiacs.umd.edu.
⁷ University of Maryland Institute of Advanced Computer Studies, University of Maryland, College Park, MD, USA. rcolwell@umiacs.umd.edu.
⁸ Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA. rcolwell@umiacs.umd.edu.

^# Contributed equally.

Abstract

Escherichia coli is a pathogen commonly encountered in clinical laboratories, and is capable of causing a variety of diseases, both within the intestinal tract (intestinal pathogenic strains) and outside (extraintestinal pathogenic E. coli, or ExPEC). It is associated with urinary tract infections (UTIs), one of the most common infectious diseases in the world. This report represents the first comparative analysis of the draft genome sequences of 11 uropathogenic E. coli (UPEC) strains isolated from two tertiary hospitals located in Dhaka and Sylhet, Bangladesh, and is focused on comparing their genomic characteristics to each other and to other available UPEC strains. Multilocus sequence typing (MLST) confirmed the strains belong to ST59, ST131, ST219, ST361, ST410, ST448 and ST4204, with one of the isolates classified as a previously undocumented ST. De novo identification of the antibiotic resistance genes bla_NDM-5, bla_NDM-7, bla_CTX-M-15 and bla_OXA-1 was determined, and phenotypic-genotypic analysis of virulence revealed significant heterogeneity within UPEC phylogroups.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bangladesh
Genotype
Multilocus Sequence Typing / methods*
Phenotype
Uropathogenic Escherichia coli / enzymology*
Uropathogenic Escherichia coli / metabolism
beta-Lactamases / metabolism*

Substances

beta-Lactamases