The isolation and genome sequencing of a novel cyanophage S-H68 from the Bohai Sea, China

Chenglong Xue; Xinxin Liu; Qi Wang; Tongtong Lin; Min Wang; Qian Liu; Hongbing Shao; Yong Jiang

doi:10.1016/j.margen.2019.100739

The isolation and genome sequencing of a novel cyanophage S-H68 from the Bohai Sea, China

Mar Genomics. 2020 Oct:53:100739. doi: 10.1016/j.margen.2019.100739. Epub 2020 Jan 2.

Authors

Chenglong Xue¹, Xinxin Liu¹, Qi Wang¹, Tongtong Lin¹, Min Wang², Qian Liu¹, Hongbing Shao¹, Yong Jiang³

Affiliations

¹ College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
² College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao 266003, China.
³ College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao 266003, China. Electronic address: yongjiang@ouc.edu.cn.

PMID: 32883437
DOI: 10.1016/j.margen.2019.100739

Abstract

Cyanobacteria, also known as bule-green algae, are capable of photosynthesis and have a fixed carbon and nitrogen effect. The virus that specifically infects cyanobacteria is called the cyanophage. Cyanophages play a key role in building microbial communities. However, only a small number of cyanophages have been reported so far. In this study, a novel Synechococcus cyanophage S-H68 was isolated from the Bohai Sea of China. Transmission electron microscope observations showed that S-H68 has an icosahedral head, 66 ± 1 nm in diameter, and a tail with a length of 107 ± 1 nm, and should be grouped into the family Siphoviridae. To better understand the genetic diversity of this cyanophage, the complete genome was characterized. It consists of 79,639 -bp -length double-stranded DNA with a GC content of 59.8% and is predicted to have 117 open reading frames (ORFs) with an average length of 655 nucleotides. Using the BLASTN tool in the NCBI database for genome comparison, there was no significant similarity between S-H68 and other known cyanophages. So the present study added a new Siphoviridae cyanophage to the marine phage dataset.

Keywords: Complete genome; Siphoviridae; Synechococcus.

MeSH terms

Bacteriophages / genetics*
China
Chromosome Mapping
Cyanobacteria / virology*
Oceans and Seas
Seawater / microbiology*
Siphoviridae / genetics*