Microbial vanadate reduction coupled to co-metabolic phenanthrene biodegradation in groundwater

Abstract

Vanadate [V(V)] and phenanthrene (PHE) commonly coexist in groundwater aquifer, posing potential threats to ecological environment and public health. However, little is known about the complicated biogeochemical processes involving microbial V(V) reduction coupled with co-metabolic PHE biodegradation. Herein we demonstrated that synchronous removal of V(V) and PHE could be realized under anaerobic condition. Complete V(V) removal and PHE degradation efficiency of 82.0 ± 0.8% were achieved in 7-d operation in batch experiment. 250-d continuous column experiment implied that hydrochemical condition affected V(V) and PHE removals. V(V) was reduced to insoluble vanadium (IV) and PHE was degraded into small molecule organics (e.g. salicylic acid). Geobacter and Acetobacterium used methanol and intermediates from PHE degradation as electron donors for V(V) reduction. PHE was decomposed by Mycobacterium and Clostridium with methanol as co-metabolic substrate and V(V) as electron acceptor. Genes encoding proteins for V(V) reduction (omcA, omcB and mtrC) and PHE degradation (phnAc) were upregulated. Cytochrome c and nicotinamide adenine dinucleotide promoted electron transfer for V(V) and PHE detoxification. Extracellular polymeric substances could bind V(V) and improve the bioavailability of PHE. Our findings provide a robust strategy for remediation of V(V) and PHE co-contaminated groundwater.

Keywords: Co-metabolism; Groundwater; Microbial evolution; Phenanthrene; Vanadate.